An Efficient Combinatorial Approach for Beta-Lactam Antibiotics with Novel Adjuvants against Gram-Negative Organisms to Combat Multi-Drug Resistance

Proceedings of the International conference on Applied Research in Engineering,Science and Technology

Year: 2018 | Page No:134-143

DOI: http://www.doi.org/10.33422/icarest.2018.09.47

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An Efficient Combinatorial Approach for Beta-Lactam Antibiotics with Novel Adjuvants against Gram-Negative Organisms to Combat Multi-Drug Resistance

Chandresh Pareek, Dushyant Pareek, Narendra Nirwan and Ajay Jain4

ABSTRACT: 

Anti-Microbial Resistance [AMR] is progressively grave menace to global wellness. New techniques and developments are almost none to least to overcome this. Herein we describe recent development toward combinatorial in-vitro therapy for the treatment of multi-drug resistant bacterial infections and we have approached to this by eliminating the overuse of antibiotics as well as by the use of an ‘antibiotic adjuvants’ in combination with an antibiotic. These attempts include the development of adjuvants that either directly target resistance mechanisms such as the inhibition of beta-lactamase enzymes or indirectly target resistance to two-component systems. To this end, we utilized and developed the anti-resistance adjuvants that cause multi-drug resistant species to become susceptible to clinical antibiotics that are no longer effective. From the initial in-vitro screening, we developed new imidazole adjuvants that decreased the effective concentration of popular beta lactam antibiotics against Pseudomonas aeruginosa and E. Coli towards a clinical multi-drug resistant. For this we calculated MIC [Minimum Inhibitory Concentration] exclusively for selected drugs and employed it with adjuvants.

Keywords: Anti-microbial Resistance; GNB, β-lactams; substituted imidazoles; MIC.