Proceedings of the International conference on Applied Research in Engineering,Science and Technology
Year: 2018 | Page No:98-110
Experimental Investigation of Extraction of Active Ingredients of Scrophularia striata Boiss (as a Medicinal Herb) via Supercritical Fluid Extraction Method with Ultrasonic Waves
Nabil vaeli ,Bizhan honarvar , Nadia esfandiari and Zahra arab.
One of the major problems faced by modern societies are various infectious diseases with different roots, most of which are supposed to be treated using chemical antibiotics. Considering side effects of these drugs and the fact that those fail to realize any positive influence in many cases, an ever increasing deal of attention has been paid to the utilization of medicinal herbs which exhibit great therapeutic properties without any side effect. This important characteristic of the medicinal herbs doubles the significance of the method used to extract active ingredients from the herbs in such a way to end up with optimal efficiency. In the present research, extraction of extract from Scrophularia striata Boiss, as a medicinal herb which is rich in active ingredients with antibacterial and anti-depression effects (isorhamnetin-3-o-rutinoside, quercetine, cinnamic acid, nepitrin), using supercritical CO2 (SC-CO2) with the help of ultrasonic waves, and also applying the same method without the use of the ultrasonic waves were investigated. Due to its low volatility, the medicinal extract in this plant is difficult to collect and extract. In the present research, it was observed that the yield of active ingredient extraction using the presented method was about 68% higher when ultrasonication was further applied, as compared to the case where no ultrasonic wave was applied. Thermodynamic conditions used in the present research included the pressure values of 150, 180, and 210 bar, and temperatures of 45, 55, and 65℃, with the ultrasonication device operated at frequencies of 37 and 80 kHz and power of 50 and 100 W. The results indicated that, when the pressure and temperature were set to 180 bar and 65℃, respectively, while USCF system was operated at 37 kHz, an output efficiency of 87% (in mass) was obtained, while the efficiency was as low as 41% under the same set of conditions but without ultrasonication.
Keywords: extraction, ultrasound, supercritical fluid, Scrophularia striata Boiss plant.