Optimization of rutin encapsulation in self-assembled polymeric particles

Proceedings of ‏The 7th International Conference on Modern Approaches in Science, Technology & Engineering

Year: 2021

DOI:

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Optimization of rutin encapsulation in self-assembled polymeric particles

Narinchote Kaiyoonwong, Natthaporn Sae-lim, and Kamolchanok Ngamkham

 

ABSTRACT: 

Rutin is a polyphenolic compound found in several fruits and vegetables. It possesses various therapeutic properties, including antioxidant, anti-inflammatory, anti-cancer, and anti-microbial. Moreover, rutin was also reported to enhance stem cell proliferation and differentiation. However, the applications of rutin are limited as it has low water solubility and low bioavailability. To overcome those limitations, in this study, rutin was encapsulated with a self-assembled amphiphilic block copolymer, poly(lactide)-b-poly(poly ethylene glycol methacrylate) (PLA-b-PPEGMA), using the solvent switch method. Different amounts of rutin and water volume have been tried and the highest encapsulation efficiency was achieved when 10 mg block copolymer was used to encapsulate 0.5 mg rutin in 5 ml water at the polymer dropping rate of 300 μl/min. The encapsulation efficiency is up to 35.04 ± 3.52 wt% and a drug loading capacity is 1.75 ± 0.176 wt% with an average particle size of 1.90 ± 0.71 µm. Nevertheless, the encapsulation efficiency and drug loading capacity can be improved by lowering the polymer dropping rate. The best condition was presented on 100 μl/min dropping rate which exhibited 65.32 ± 11.69% encapsulation and 3.27 ± 0.58% drug loading capacity, respectively. Moreover, the release of rutin from the particles was sustained for more than 4 hours, while free rutin reached 100% released after 45 minutes. The release mechanism was most fitted to the Weibull model with a shape parameter indicating a complex release mechanism. This research optimized a drug encapsulation protocol for making a micro-scale rutin delivery carrier which can be utilized with further drug delivery applications.

Keywords: diblock copolymer; drug release; particles; rutin encapsulation; self-assembly.