Proceedings of the 7th International Conference on Academic Research in Science, Technology and Engineering
Year: 2025
DOI:
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Biosurfactant production and Oil biodegradation by marine bacteria isolated from Red Sea, Saudi Arabia
Raghad Obaid Alzahrani, Nada Hassan Doleib and Nashwa Ibrahem Hagagy
ABSTRACT:
The current study aimed to determine the capacity of marine bacterial isolates to produce biosurfactant by using crude oil as the sole carbon source. The isolates were screened for biosurfactant production using emulsification activity, drop collapsing test and oil displacement test. 16S rRNA gene sequencing was performed and the results were deposited to the NCBI GenBank database under the accession numbers PP434619 and PP434620. The tested isolates were identified as vreelandella aquamarina strain SNP1, Halomonas sp strain SNP2 were found to produce biosurfactants due to the halo-emulsified that surrounded the colonies. To obtain further information regarding the biochemical characterization, the polymeric extract was analyzed using gas chromatography-mass spectrometry (GC-MS), identified 20 distinct constituents as Gallic acid, Chlorogenic acid, Catechin, Methyl gallate, Caffeic acid, Syringic acid, Rutin, Ellagic acid, Coumaric acid, Vanillin, Ferulic acid, Naringenin, Rosmarinic acid, Daidzein, Quercetin, Cinnamic acid, Kaempferol, Hesperetin. These findings provide important information indicating the metabolic capabilities of the studied isolates regarding hydrocarbon degradation and biosurfactant production.
keywords: Biosurfactant, Hydrocarbon, Biodegradation, GC-MASS, Marine bacteria