Proceedings of The 7th International Conference on Innovation in Science and Technology
Applying Reduced Graphene Oxide-Gold Nanoparticles for the Electrochemical Detection of Endotoxin
Fatemeh Yazdian, Hamid Rashedi
The outer membrane of gram-negative bacteria releases lipopolysaccharides (LPS) or endotoxins that make toxic effects on humans. Therefore, the selective detection and sensitivity of LPS is so vital especially in the field of therapeutics, medical supplies, and in the food industry. In this study, in order to detection of LPS an ultrasensitive electrochemical biosensor was fabricated through the immobilization of endotoxin-specific thiolated aptamer on the surface of reduced graphene oxide-gold nanoparticles (rGO-Au NPs) modified electrode. The biosensor is found to detect the small amounts of LPS in 35 minutes and was able to distinguish the endotoxin molecules from the interfering biomolecules such as bovine serum albumin, fetal bovine serum, and glucose. Hence, this sensor has excellent sensitivity and selectivity toward LPS, in spite of its convenient and easy construction procedure. the Real sample test proved that patient group has higher ∆I (higher than 55 mA) comparing with the normal group (less than 55 mA).
Keywords: Endotoxin; Aptasensor; Gold nanoparticles; Lipopolysaccharide; Methylene blue.