Proceedings of The 7th International Conference on Innovation in Science and Technology
Year: 2020
DOI:
Optimizing Electrospinning Parameters of Polymeric Scaffold Using Anfis for Amd Treatment
Amin Janghorbani, Saleheh Shahmoradi and Fatemeh Yazdian
ABSTRACT:
Among wide range application of stem cells in tissue engineering, using them in Age-related macular degeneration (AMD) has recently payed attention. AMD is one of the retinal degenerative diseases associated with some degree of dysfunction and loss of retinal pigmented epithelium (RPE) cells and leads to permanent sight loss. In this study, ANFIS was used for optimization the electrospinning’s parameters to make scaffold. For better adhesion and proliferation of cells, the polycaprolactone scaffold’s surface was modified by alkaline hydrolysis. Some analyses, such as water uptake and biodegradation rate were done. Then, differentiated human embryonic stem cells (hESCs) were cultured on several groups of scaffolds. Finally, viability, proliferation, and morphology of differentiated hESC-RPE cells on all groups of the scaffolds were investigated. The nanofibers’ diameter was minimized by optimization of voltage and solution concentration with fuzzy model for the first time, which obtained 110.5 nm, 19.1 kV, and 0.065 g/mL (w/v), respectively. Immersion time of scaffold in alkaline solution and concentration of solution during surface modification were achieved 104 minutes and 4.3 M, respectively. Results of the MTT assay showed that the hydrolyzed group had a high proliferation of cells. Scanning electron microscopy observation of cell morphology after two months confirmed this result. In conclusion, our results demonstrate that the hydrolyzed scaffold is a suitable bed for cell proliferation, which can be a good option for AMD treatment.
Keywords: ANFIS, Age-related macular degeneration, Polycaprolactone, Optimization, hESCs, Fuzzy model.