Proceedings of The 7th International Conference on Innovation in Science and Technology
Wound Dressing Based on PVA
Mohammad Ali Salmeh, Melika Mohammadi Yazdi, Fatemeh Yazdian, Hamid Rashedi
The number of patients with various types of skin wounds has increased due to the increase in population and the emergence of new skin diseases. So that other traditional dressings and techniques have fallen out of the reach of researchers and physicians and replaced with new ones. Nowadays, biopolymer polymeric scaffolds, especially nanofibers, are widely considered for engineering applications of skin tissue and wound coating. At first, GO was synthesized from graphite and then added to the extract of Nigella sativa and Arginine. FTIR, Zeta potential and SEM analysis were used to confirm the accuracy of the materials. The antibacterial properties of the materials were also evaluated by MIC, OD and Halo assay. The results showed that these materials are capable of inhibiting the growth of both groups of bacteria, Gram-positive and Gram-negative. Next, the polyvinyl alcohol solution was prepared and combined with Graphene oxide -Nigella sativa – Arginine to produce by the electro spinning process of nanofibers. FTIR analysis confirmed the presence of nanomaterials in the nanofibers produced. NIH 3T3 fibroblast cells were used to evaluate cell toxicity and viability by MTT method. The results indicate the non-toxicity of Graphene oxide -Nigella sativa -Arginine nanocomposite at concentration 2 micrograms per mL.
Keywords: Wound dressing; Nigella sativa; Graphene oxide; Arginine; Polyvinyl alcohol.